How Does Disruption of Circatidal Clock Protein Effect Juvenile Horseshoe Crabs

Recent research in the field of biological clocks shows that perA, perB and perC genes code for key proteins involved in the negative feedback loop of circadian clocks in Limulus polyphemus. This study addresses whether or not these isoforms of the period gene play a critical role in another biological clock of the American Horseshoe crab, the circatidal clock. Utilizing microinjection, a dsRNA cocktail of perA, perB and perC genes was introduced into juvenile horseshoe crabs. Ethovision X13 software compiled video data for later analysis within the Clocklab application that would produce actograms and periodograms. An ANOVA was performed among the treatment groups after injection and resulted in a P-value of 0.0234. This statistical significance suggests the targeted isoforms of the per gene are significant factors in the molecular clock that drives the circatidal rhythm in this species.

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